Sensitivity of the airway smooth muscle in terms of force, shortening and stiffness.
Respir Physiol Neurobiol
; 325: 104264, 2024 Jul.
Article
en En
| MEDLINE
| ID: mdl-38599345
ABSTRACT
Eight pig tracheal strips were stimulated to contract with log increments of methacholine from 10-8 to 10-5 M. For each strip, the concentration-response was repeated four times in a randomized order to measure isometric force, isotonic shortening against a load corresponding to either 5 or 10â¯% of a reference force, and average force, stiffness, elastance and resistance over one cycle while the strip length was oscillating sinusoidally by 5â¯% at 0.2â¯Hz. For each readout, the logEC50 was calculated and compared. Isotonic shortening with a 5â¯% load had the lowest logEC50 (-7.13), yielding a greater sensitivity than any other contractile readout (p<0.05). It was followed by isotonic shortening with a 10â¯% load (-6.66), elastance (-6.46), stiffness (-6.46), resistance (-6.38), isometric force (-6.32), and average force (-6.30). Some of these differences were significant. For example, the EC50 with the average force was 44â¯% greater than with the elastance (p=0.001). The methacholine sensitivity is thus affected by the contractile readout being measured.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Tráquea
/
Broncoconstrictores
/
Cloruro de Metacolina
/
Músculo Liso
Límite:
Animals
Idioma:
En
Revista:
Respir Physiol Neurobiol
Año:
2024
Tipo del documento:
Article