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Neural Tracing Protein-Functionalized Nanoparticles Capable of Fast Retrograde Axonal Transport in Live Neurons.
Wang, Wenqian; Hassan, Md Musfizur; Kapoor-Kaushik, Natasha; Livni, Lital; Musrie, Benjamin; Tang, Jianbo; Mahmud, Zaheri; Lai, Saluo; Wich, Peter Richard; Ananthanarayanan, Vaishnavi; Moalem-Taylor, Gila; Mao, Guangzhao.
Afiliación
  • Wang W; School of Chemical Engineering, University of New South Wales (UNSW Sydney), Sydney, NSW, 2052, Australia.
  • Hassan MM; School of Chemical Engineering, University of New South Wales (UNSW Sydney), Sydney, NSW, 2052, Australia.
  • Kapoor-Kaushik N; Electron Microscopy Unit, University of New South Wales (UNSW), Sydney, NSW, 2052, Australia.
  • Livni L; School of Biomedical Sciences, University of New South Wales (UNSW), Sydney, NSW, 2052, Australia.
  • Musrie B; School of Biomedical Sciences, University of New South Wales (UNSW), Sydney, NSW, 2052, Australia.
  • Tang J; School of Chemical Engineering, University of New South Wales (UNSW Sydney), Sydney, NSW, 2052, Australia.
  • Mahmud Z; School of Chemical Engineering, University of New South Wales (UNSW Sydney), Sydney, NSW, 2052, Australia.
  • Lai S; School of Chemical Engineering, University of New South Wales (UNSW Sydney), Sydney, NSW, 2052, Australia.
  • Wich PR; School of Chemical Engineering, University of New South Wales (UNSW Sydney), Sydney, NSW, 2052, Australia.
  • Ananthanarayanan V; EMBL Australia Node in Single Molecule Science, Department of Molecular Medicine, School of Biomedical Sciences, University of New South Wales (UNSW), Sydney, NSW, 2052, Australia.
  • Moalem-Taylor G; School of Biomedical Sciences, University of New South Wales (UNSW), Sydney, NSW, 2052, Australia.
  • Mao G; School of Chemical Engineering, University of New South Wales (UNSW Sydney), Sydney, NSW, 2052, Australia.
Small ; 20(39): e2311921, 2024 Sep.
Article en En | MEDLINE | ID: mdl-38647340
ABSTRACT
Neural tracing proteins like horseradish peroxidase-conjugated wheat germ agglutinin (WGA-HRP) can target the central nervous system (CNS) through anatomic retrograde transport without crossing the blood-brain barrier (BBB). Conjugating WGA-HRP to nanoparticles may enable the creation of BBB-bypassing nanomedicine. Microfluidics and two-photon confocal microscopy is applied to screen nanocarriers for transport efficacy and gain mechanistic insights into their interactions with neurons. Protein modification of gold nanoparticles alters their cellular uptake at the axonal terminal and activates fast retrograde transport. Trajectory analysis of individual endosomes carrying the nanoparticles reveals a run-and-pause pattern along the axon with endosomes carrying WGA-HRP-conjugated gold nanoparticles exhibiting longer run duration and faster instantaneous velocity than those carrying nonconjugated nanoparticles. The results offer a mechanistic explanation of the different axonal transport dynamics as well as a cell-based functional assay of neuron-targeted nanoparticles with the goal of developing BBB-bypassing nanomedicine for the treatment of nervous system disorders.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Transporte Axonal / Oro / Neuronas Límite: Animals Idioma: En Revista: Small Asunto de la revista: ENGENHARIA BIOMEDICA Año: 2024 Tipo del documento: Article País de afiliación: Australia
Texto completo
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Transporte Axonal / Oro / Neuronas Límite: Animals Idioma: En Revista: Small Asunto de la revista: ENGENHARIA BIOMEDICA Año: 2024 Tipo del documento: Article País de afiliación: Australia