UBE2C promotes myoblast differentiation and skeletal muscle regeneration through the Akt signaling pathway.
Acta Biochim Biophys Sin (Shanghai)
; 56(7): 1065-1071, 2024 Apr 29.
Article
en En
| MEDLINE
| ID: mdl-38690615
ABSTRACT
Ubiquitin-conjugation enzyme E2C (UBE2C) is a crucial component of the ubiquitin-proteasome system that is involved in numerous cancers. In this study, we find that UBE2C expression is significantly increased in mouse embryos, a critical stage during skeletal muscle development. We further investigate the function of UBE2C in myogenesis. Knockdown of UBE2C inhibits C2C12 cell differentiation and decreases the expressions of MyoG and MyHC, while overexpression of UBE2C promotes C2C12 cell differentiation. Additionally, knockdown of UBE2C, specifically in the tibialis anterior muscle (TA), severely impedes muscle regeneration in vivo. Mechanistically, we show that UBE2C knockdown reduces the level of phosphorylated protein kinase B (p-Akt) and promotes the degradation of Akt. These findings suggest that UBE2C plays a critical role in myoblast differentiation and muscle regeneration and that UBE2C regulates myogenesis through the Akt signaling pathway.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Regeneración
/
Transducción de Señal
/
Diferenciación Celular
/
Músculo Esquelético
/
Desarrollo de Músculos
/
Mioblastos
/
Enzimas Ubiquitina-Conjugadoras
/
Proteínas Proto-Oncogénicas c-akt
Límite:
Animals
Idioma:
En
Revista:
Acta Biochim Biophys Sin (Shanghai)
Asunto de la revista:
BIOFISICA
/
BIOQUIMICA
Año:
2024
Tipo del documento:
Article
País de afiliación:
China