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Development of a thermotaxis and rheotaxis microfluidic device for motile spermatozoa sorting.
Huang, Teng-Kuan; Huang, Chung-Hsien; Chen, Pei-An; Chen, Ching Hung; Lu, Farn; Yang, Wen-Ju; Huang, Jack Yu Jen; Li, Bor-Ran.
Afiliación
  • Huang TK; Institute of Biomedical Engineering, College of Electrical and Computer Engineering, National Yang Ming Chiao Tung University, Hsinchu, Taiwan.
  • Huang CH; Institute of Biomedical Engineering, College of Electrical and Computer Engineering, National Yang Ming Chiao Tung University, Hsinchu, Taiwan; Department of Electrical and Computer Engineering, College of Electrical and Computer Engineering, National Yang Ming Chiao Tung University, Hsinchu, Taiwan
  • Chen PA; Institute of Biomedical Engineering, College of Electrical and Computer Engineering, National Yang Ming Chiao Tung University, Hsinchu, Taiwan; Department of Electrical and Computer Engineering, College of Electrical and Computer Engineering, National Yang Ming Chiao Tung University, Hsinchu, Taiwan
  • Chen CH; Taiwan IVF Group, Hsinchu, Taiwan; Ton Yen General Hospital, Hsinchu, Taiwan.
  • Lu F; Taiwan IVF Group, Hsinchu, Taiwan; Ton Yen General Hospital, Hsinchu, Taiwan.
  • Yang WJ; Taiwan IVF Group, Hsinchu, Taiwan; Ton Yen General Hospital, Hsinchu, Taiwan.
  • Huang JYJ; Taiwan IVF Group, Hsinchu, Taiwan; Ton Yen General Hospital, Hsinchu, Taiwan; Division of Reproductive Endocrinology & Infertility, The Department of Obstetrics and Gynecology at Stanford University, Stanford, CA, USA.
  • Li BR; Institute of Biomedical Engineering, College of Electrical and Computer Engineering, National Yang Ming Chiao Tung University, Hsinchu, Taiwan; Department of Electrical and Computer Engineering, College of Electrical and Computer Engineering, National Yang Ming Chiao Tung University, Hsinchu, Taiwan
Biosens Bioelectron ; 258: 116353, 2024 Aug 15.
Article en En | MEDLINE | ID: mdl-38696966
ABSTRACT
Male infertility is a pervasive global reproductive challenge, primarily attributed to a decline in semen quality. Addressing this concern, there has been a growing focus on spermatozoa sorting in assisted reproductive technology. This study introduces a groundbreaking development in the form of a thermotaxis and rheotaxis microfluidic (TRMC) device designed for efficient motile spermatozoa sorting within a short 15-min timeframe. The TRMC device mimics the natural sperm sorting mechanism of the oviduct, selecting spermatozoa with superior motility and DNA integrity. The experimental outcomes demonstrate a remarkable enhancement in the percentage of progressive spermatozoa following sorting, soaring from 3.90% to an impressive 96.11% when subjected to a temperature decrease from 38 °C to 35 °C. Notably, sperm motility exhibited a substantial 69% improvement. The TRMC device exhibited a commendable recovery rate of 60.93%, surpassing current clinical requirements. Furthermore, the sorted spermatozoa displayed a notable reduction in the DNA fragmentation index to 6.94%, signifying a substantial 90% enhancement in DNA integrity. This remarkable advancement positions the TRMC device as highly suitable for applications in in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI), offering a promising solution to male infertility challenges.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Motilidad Espermática / Espermatozoides / Dispositivos Laboratorio en un Chip Límite: Humans / Male Idioma: En Revista: Biosens Bioelectron Asunto de la revista: BIOTECNOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: Taiwán
Texto completo
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Motilidad Espermática / Espermatozoides / Dispositivos Laboratorio en un Chip Límite: Humans / Male Idioma: En Revista: Biosens Bioelectron Asunto de la revista: BIOTECNOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: Taiwán