Microwave-assisted and methanol/acetic acid-free method for rapid staining of proteins in SDS-PAGE gels.

Wang, Jinzhong; Wang, Zhaoyang; Zhao, Wei; Wang, Ying

Wang, Jinzhong; Wang, Zhaoyang; Zhao, Wei; Wang, Ying.

Afiliación

Wang J; TEDA Institute of Biological Sciences and Biotechnology, Nankai University, 23 Hongda Street, TEDA, Tianjin, 300457, China; Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, 23 Hongda Street, TEDA, Tianjin, 300457, China; Tianjin Key Laboratory of Microbial Functional G
Wang Z; TEDA Institute of Biological Sciences and Biotechnology, Nankai University, 23 Hongda Street, TEDA, Tianjin, 300457, China.
Zhao W; TEDA Institute of Biological Sciences and Biotechnology, Nankai University, 23 Hongda Street, TEDA, Tianjin, 300457, China.
Wang Y; TEDA Institute of Biological Sciences and Biotechnology, Nankai University, 23 Hongda Street, TEDA, Tianjin, 300457, China; Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, 23 Hongda Street, TEDA, Tianjin, 300457, China; Tianjin Key Laboratory of Microbial Functional G

Anal Biochem ; 691: 115553, 2024 Aug.

Article en En | MEDLINE | ID: mdl-38697592

ABSTRACT

ABSTRACT

We describe a microwave-assisted, methanol and acetic acid-free, inexpensive method for rapid staining of SDS-PAGE proteins. Only citric acid, benzoic acid, and Coomassie brilliant blue G-250 (CBG) were used. Microwave irradiation reduced the detection duration, and proteins in a clear background were visualized within 30 min of destaining, after 2 min of fixing and 12 min of staining. By using this protocol, comparable band intensities were obtained to the conventional methanol/acetic acid method.

Asunto(s)

Ácido Acético; Electroforesis en Gel de Poliacrilamida; Metanol; Microondas; Proteínas; Electroforesis en Gel de Poliacrilamida/métodos; Metanol/química; Proteínas/análisis; Ácido Acético/química; Coloración y Etiquetado/métodos; Colorantes de Rosanilina/química

Palabras clave

CBG stain; Citric acid; Coomassie brilliant blue G-250; Methanol/acetic acid-free; Microwave-assisted

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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Proteínas / Ácido Acético / Metanol / Electroforesis en Gel de Poliacrilamida / Microondas Idioma: En Revista: Anal Biochem / Anal. biochem / Analytical biochemistry Año: 2024 Tipo del documento: Article

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