AlbuMAX supplemented media induces the formation of transmission-competent P. falciparum gametocytes.
Mol Biochem Parasitol
; 259: 111634, 2024 09.
Article
en En
| MEDLINE
| ID: mdl-38823647
ABSTRACT
Asexual blood stage culture of Plasmodium falciparum is routinely performed but reproducibly inducing commitment to and maturation of viable gametocytes remains difficult. Culture media can be supplemented with human serum substitutes to induce commitment but these generally only allow for long-term culture of asexual parasites and not transmission-competent gametocytes due to their different lipid composition. Recent insights demonstrated the important roles lipids play in sexual commitment; elaborating on this we exposed ring stage parasites (20-24â¯hours hpi) for one day to AlbuMAX supplemented media to trigger induction to gametocytogenesis. We observed a significant increase in gametocytes after AlbuMAX induction compared to serum. We also tested the transmission potential of AlbuMAX inducted gametocytes and found a significant higher oocyst intensity compared to serum. We conclude that AlbuMAX supplemented media induces commitment, allows a more stable and predictable production of transmittable gametocytes than serum alone.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Plasmodium falciparum
/
Medios de Cultivo
Límite:
Humans
Idioma:
En
Revista:
Mol Biochem Parasitol
Año:
2024
Tipo del documento:
Article
País de afiliación:
Países Bajos