Predicting Rubisco-Linker Condensation from Titration in the Dilute Phase.
Phys Rev Lett
; 132(21): 218401, 2024 May 24.
Article
en En
| MEDLINE
| ID: mdl-38856270
ABSTRACT
The condensation of Rubisco holoenzymes and linker proteins into "pyrenoids," a crucial supercharger of photosynthesis in algae, is qualitatively understood in terms of "sticker-and-spacer" theory. We derive semianalytical partition sums for small Rubisco-linker aggregates, which enable the calculation of both dilute-phase titration curves and dimerization diagrams. By fitting the titration curves to surface plasmon resonance and single-molecule fluorescence microscopy data, we extract the molecular properties needed to predict dimerization diagrams. We use these to estimate typical concentrations for condensation, and successfully compare these to microscopy observations.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Idioma:
En
Revista:
Phys Rev Lett
Año:
2024
Tipo del documento:
Article
País de afiliación:
Reino Unido