Your browser doesn't support javascript.
loading
descSPIM: an affordable and easy-to-build light-sheet microscope optimized for tissue clearing techniques.
Otomo, Kohei; Omura, Takaki; Nozawa, Yuki; Edwards, Steven J; Sato, Yukihiko; Saito, Yuri; Yagishita, Shigehiro; Uchida, Hitoshi; Watakabe, Yuki; Naitou, Kiyotada; Yanai, Rin; Sahara, Naruhiko; Takagi, Satoshi; Katayama, Ryohei; Iwata, Yusuke; Shiokawa, Toshiro; Hayakawa, Yoku; Otsuka, Kensuke; Watanabe-Takano, Haruko; Haneda, Yuka; Fukuhara, Shigetomo; Fujiwara, Miku; Nii, Takenobu; Meno, Chikara; Takeshita, Naoki; Yashiro, Kenta; Rosales Rocabado, Juan Marcelo; Kaku, Masaru; Yamada, Tatsuya; Oishi, Yumiko; Koike, Hiroyuki; Cheng, Yinglan; Sekine, Keisuke; Koga, Jun-Ichiro; Sugiyama, Kaori; Kimura, Kenichi; Karube, Fuyuki; Kim, Hyeree; Manabe, Ichiro; Nemoto, Tomomi; Tainaka, Kazuki; Hamada, Akinobu; Brismar, Hjalmar; Susaki, Etsuo A.
Afiliación
  • Otomo K; Department of Biochemistry and Systems Biomedicine, Juntendo University Graduate School of Medicine, Tokyo, Japan.
  • Omura T; Biochemistry II, Juntendo University School of Medicine, Tokyo, Japan.
  • Nozawa Y; Nakatani Biomedical Spatialomics Hub, Juntendo University Graduate School of Medicine, Tokyo, Japan.
  • Edwards SJ; Division of Biophotonics, National Institute for Physiological Sciences, National Institutes of Natural Sciences, Okazaki, Japan.
  • Sato Y; Biophotonics Research Group, Exploratory Research Center on Life and Living Systems, National Institutes of Natural Sciences, Okazaki, Japan.
  • Saito Y; Department of Biochemistry and Systems Biomedicine, Juntendo University Graduate School of Medicine, Tokyo, Japan.
  • Yagishita S; Nakatani Biomedical Spatialomics Hub, Juntendo University Graduate School of Medicine, Tokyo, Japan.
  • Uchida H; Department of Neurosurgery, University of Tokyo, Tokyo, Japan.
  • Watakabe Y; Department of Neurosurgery and Neuro-Oncology, National Cancer Center Hospital, Tokyo, Japan.
  • Naitou K; Biochemistry II, Juntendo University School of Medicine, Tokyo, Japan.
  • Yanai R; Science for Life Laboratory, Department of Applied Physics, KTH Royal Institute of Technology, Stockholm, Sweden.
  • Sahara N; Department of Biochemistry and Systems Biomedicine, Juntendo University Graduate School of Medicine, Tokyo, Japan.
  • Takagi S; Nakatani Biomedical Spatialomics Hub, Juntendo University Graduate School of Medicine, Tokyo, Japan.
  • Katayama R; Department of Biochemistry and Systems Biomedicine, Juntendo University Graduate School of Medicine, Tokyo, Japan.
  • Iwata Y; Nakatani Biomedical Spatialomics Hub, Juntendo University Graduate School of Medicine, Tokyo, Japan.
  • Shiokawa T; Department of Pharmacology and Therapeutics, Fundamental Innovative Oncology Core, National Cancer Center Research Institute, Tokyo, Japan.
  • Hayakawa Y; Division of Molecular Pharmacology, National Cancer Center Research Institute, Tokyo, Japan.
  • Otsuka K; Department of System Pathology for Neurological Disorders, Brain Research Institute, Niigata University, Niigata, Japan.
  • Watanabe-Takano H; Division of Biophotonics, National Institute for Physiological Sciences, National Institutes of Natural Sciences, Okazaki, Japan.
  • Haneda Y; Biophotonics Research Group, Exploratory Research Center on Life and Living Systems, National Institutes of Natural Sciences, Okazaki, Japan.
  • Fukuhara S; Department of Basic Veterinary Science, Joint Faculty of Veterinary Medicine, Kagoshima University, Kagoshima, Japan.
  • Fujiwara M; Advanced Neuroimaging Center, Institute for Quantum Medical Sciences, National Institutes for Quantum Science and Technology, Chiba, Japan.
  • Nii T; Advanced Neuroimaging Center, Institute for Quantum Medical Sciences, National Institutes for Quantum Science and Technology, Chiba, Japan.
  • Meno C; Division of Experimental Chemotherapy, Cancer Chemotherapy Center, Japanese Foundation for Cancer Research, Tokyo, Japan.
  • Takeshita N; Division of Experimental Chemotherapy, Cancer Chemotherapy Center, Japanese Foundation for Cancer Research, Tokyo, Japan.
  • Yashiro K; Department of Gastroenterology, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.
  • Rosales Rocabado JM; Department of Gastroenterology, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.
  • Kaku M; Department of Gastroenterology, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.
  • Yamada T; Biology and Environmental Chemistry Division, Sustainable System Research Laboratory, Central Research Institute of Electric Power Industry, Chiba, Japan.
  • Oishi Y; Department of Molecular Pathophysiology, Institute for Advanced Medical Sciences, Nippon Medical School, Tokyo, Japan.
  • Koike H; Department of Molecular Pathophysiology, Institute for Advanced Medical Sciences, Nippon Medical School, Tokyo, Japan.
  • Cheng Y; Department of Molecular Pathophysiology, Institute for Advanced Medical Sciences, Nippon Medical School, Tokyo, Japan.
  • Sekine K; Department of Developmental Biology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan.
  • Koga JI; Department of Developmental Biology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan.
  • Sugiyama K; Department of Developmental Biology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan.
  • Kimura K; Anatomy and Developmental Biology, Kyoto Prefectural University of Medicine, Kyoto, Japan.
  • Karube F; Department of Pediatrics, Kyoto Prefectural University of Medicine, Kyoto, Japan.
  • Kim H; Anatomy and Developmental Biology, Kyoto Prefectural University of Medicine, Kyoto, Japan.
  • Manabe I; Division of Bio-prosthodontics, Faculty of Dentistry & Graduate School of Medical and Dental Sciences, Niigata University, Niigata, Japan.
  • Nemoto T; Division of Bio-prosthodontics, Faculty of Dentistry & Graduate School of Medical and Dental Sciences, Niigata University, Niigata, Japan.
  • Tainaka K; Department of Biochemistry, University of Nebraska-Lincoln, Lincoln, USA.
  • Hamada A; Department of Meidical Biochemistry, Tokyo Medical and Dental University, Tokyo, Japan.
  • Brismar H; Department of Meidical Biochemistry, Tokyo Medical and Dental University, Tokyo, Japan.
  • Susaki EA; Department of Meidical Biochemistry, Tokyo Medical and Dental University, Tokyo, Japan.
Nat Commun ; 15(1): 4941, 2024 Jun 12.
Article en En | MEDLINE | ID: mdl-38866781
ABSTRACT
Despite widespread adoption of tissue clearing techniques in recent years, poor access to suitable light-sheet fluorescence microscopes remains a major obstacle for biomedical end-users. Here, we present descSPIM (desktop-equipped SPIM for cleared specimens), a low-cost ($20,000-50,000), low-expertise (one-day installation by a non-expert), yet practical do-it-yourself light-sheet microscope as a solution for this bottleneck. Even the most fundamental configuration of descSPIM enables multi-color imaging of whole mouse brains and a cancer cell line-derived xenograft tumor mass for the visualization of neurocircuitry, assessment of drug distribution, and pathological examination by false-colored hematoxylin and eosin staining in a three-dimensional manner. Academically open-sourced ( https//github.com/dbsb-juntendo/descSPIM ), descSPIM allows routine three-dimensional imaging of cleared samples in minutes. Thus, the dissemination of descSPIM will accelerate biomedical discoveries driven by tissue clearing technologies.
Asunto(s)
Texto completo
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Encéfalo / Imagenología Tridimensional / Microscopía Fluorescente Límite: Animals / Humans Idioma: En Revista: Nat Commun Asunto de la revista: BIOLOGIA / CIENCIA Año: 2024 Tipo del documento: Article País de afiliación: Japón
Texto completo
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Encéfalo / Imagenología Tridimensional / Microscopía Fluorescente Límite: Animals / Humans Idioma: En Revista: Nat Commun Asunto de la revista: BIOLOGIA / CIENCIA Año: 2024 Tipo del documento: Article País de afiliación: Japón