Protocol for whole-mount immunofluorescence staining of ECM gel-embedded innervated pancreatic organoids.
STAR Protoc
; 5(2): 103132, 2024 Jun 21.
Article
en En
| MEDLINE
| ID: mdl-38875112
ABSTRACT
The mandatory usage of extracellular matrix (ECM) gels in 3D cultures limits antibody penetration and increases background, while the removal of ECM gel causes disruption of morphology and sample loss. These factors pose challenges to effective immune labeling-based staining. Here, we present a protocol for whole-mount immunofluorescence staining of gel-embedded pancreatic organoids. We describe steps for sample fixation, blocking, and antibody incubation. We detail procedures for washing antibodies and mounting.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Páncreas
/
Organoides
/
Técnica del Anticuerpo Fluorescente
/
Matriz Extracelular
Límite:
Animals
/
Humans
Idioma:
En
Revista:
STAR Protoc
Año:
2024
Tipo del documento:
Article
País de afiliación:
Turquía