Impact of hepatocyte growth factor on the colonic morphology and gut microbiome in short bowel syndrome rat model.
Pediatr Surg Int
; 40(1): 185, 2024 Jul 13.
Article
en En
| MEDLINE
| ID: mdl-38997605
ABSTRACT
PURPOSE:
This study aimed to investigate the impact of hepatocyte growth factor (HGF) on colonic morphology and gut microbiota in a rat model of short bowel syndrome (SBS).METHODS:
SD rats underwent jugular vein catheterization for total parenteral nutrition (TPN) and 90% small bowel resection [TPN + SBS (control group) or TPN + SBS + intravenous HGF (0.3 mg/kg/day, HGF group)]. Rats were harvested on day 7. Colonic morphology, gut microflora, tight junction, and Toll-like receptor-4 (TLR4) were evaluated.RESULTS:
No significant differences were observed in the colonic morphological assessment. No significant differences were observed in the expression of tight junction-related genes in the proximal colon. However, the claudin-1 expression tended to increase and the claudin-3 expression tended to decrease in the distal colon of the HGF group. The Verrucomicrobiota in the gut microflora of the colon tended to increase in the HGF group. The abundance of most LPS-producing microbiota was lower in the HGF group than in the control group. The gene expression of TLR4 was significantly downregulated in the distal colon of the HGF group.CONCLUSION:
HGF may enhance the mucus barrier through the tight junctions or gut microbiome in the distal colon.Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Síndrome del Intestino Corto
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Ratas Sprague-Dawley
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Factor de Crecimiento de Hepatocito
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Colon
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Modelos Animales de Enfermedad
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Microbioma Gastrointestinal
Límite:
Animals
Idioma:
En
Revista:
Pediatr Surg Int
Asunto de la revista:
PEDIATRIA
Año:
2024
Tipo del documento:
Article
País de afiliación:
Japón