Tlx3 mediates neuronal differentiation and proper condensation of the developing trigeminal ganglion.
Dev Biol
; 515: 79-91, 2024 Nov.
Article
en En
| MEDLINE
| ID: mdl-39019425
ABSTRACT
The trigeminal ganglion, the largest of the vertebrate cranial ganglia, is comprised of sensory neurons that relay sensations of pain, touch, and temperature to the brain. These neurons are derived from two embryonic cell types, the neural crest and ectodermal placodes, whose interactions are critical for proper ganglion formation. While the T-cell leukemia homeobox 3 (Tlx3) gene is known to be expressed in placodally-derived sensory neurons and necessary for their differentiation, little was known about Tlx3 expression and/or function in the neural crest-derived component of the developing trigeminal ganglion. By combining lineage labeling with in situ hybridization in the chick embryo, we show that neural crest-derived cells that contribute to the cranial trigeminal ganglion express Tlx3 at a time point that coincides with the onset of ganglion condensation. Importantly, loss of Tlx3 function in vivo diminishes the overall size and abundance of neurons within the trigeminal ganglion. Conversely, ectopic expression of Tlx3 in migrating cranial neural crest results in their premature neuronal differentiation. Taken together, our results demonstrate a critical role for Tlx3 in neural crest-derived cells during chick trigeminal gangliogenesis.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Diferenciación Celular
/
Ganglio del Trigémino
/
Proteínas de Homeodominio
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Regulación del Desarrollo de la Expresión Génica
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Cresta Neural
Límite:
Animals
Idioma:
En
Revista:
Dev Biol
Año:
2024
Tipo del documento:
Article
País de afiliación:
Estados Unidos