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Antiviral and cytotoxic activities and chemical profiles of two species of Abies nordmanniana from Türkiye.
Sarikahya, Nazli; Sümer Okkali, Gaye; Çöven, Furkan O; Zeinali, Sevda; Gucur, Gülce; Çaglar, A Dila; Uyar, Ece; Gören, Ahmet C; Nalbantsoy, Ayse.
Afiliación
  • Sarikahya N; Department of Chemistry, Faculty of Science, Ege University, Izmir, Turkiye.
  • Sümer Okkali G; Department of Chemistry, Faculty of Science, Ege University, Izmir, Turkiye.
  • Çöven FO; Department of Bioengineering, Faculty of Engineering, Ege University, Izmir, Turkiye.
  • Zeinali S; Department of Biotechnology, Faculty of Science, Ege University, Izmir, Turkiye.
  • Gucur G; Department of Chemistry, Faculty of Science, Ege University, Izmir, Turkiye.
  • Çaglar AD; Department of Bioengineering, Faculty of Engineering, Ege University, Izmir, Turkiye.
  • Uyar E; Department of Bioengineering, Faculty of Engineering, Ege University, Izmir, Turkiye.
  • Gören AC; Department of Chemistry, Faculty of Basic Sciences, Gebze Technical University, Kocaeli, Turkiye.
  • Nalbantsoy A; Department of Bioengineering, Faculty of Engineering, Ege University, Izmir, Turkiye.
Turk J Chem ; 48(3): 436-447, 2024.
Article en En | MEDLINE | ID: mdl-39050931
ABSTRACT
Abies is an important genus of the family Pinaceae, with about 50 species found in the highlands of Asia, Europe, North Africa, and North and Central America. The principal aim of the present work was to investigate the chemical content and biological potential of the resin and cone from Abies nordmanniana subsp. bornmulleriana and Abies nordmanniana subsp. equi-trojani, respectively. The flavonoid and phenolic contents of the resin and cones were evaluated using liquid chromatography-high resolution mass spectrometry (LC-HRMS). Additionally, the essential oil and fatty acid compositions were analyzed using gas chromatography-mass spectrometry (GC-MS) and gas chromatography-flame ionization detector (GC-FID), respectively. Cytotoxicity of the extracts and essential oils were screened against certain cancer cell lines, namely, human prostate adenocarcinoma cell line (PC3), human lung adenocarcinoma cell line (A549), human pancreatic cancer cell line (PANC-1), human hepatocellular carcinoma cell line (HepG2), human breast cancer cell line (MDA-MB231), and normal human lung fibroblast cell line (CCD-34-LU), with MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide] assay. According to the MTT results, hexane extracts of both cone (CH) and resin (RH), ethanol-water (CEW), dichloromethane (CD), and acetone (CA) extracts of the cone mostly inflict cytotoxicity in HepG2 cell line. Antiviral activities of Abies nordmanniana subsp. extracts at doses of 5 µg/g and 10 µg/g were also evaluated in ovo for their virucidal activity against avian coronavirus. Abies nordmanniana subsp. extracts exhibited concentration-dependent antiviral activity on specific pathogen-free embryonated chicken eggs. Significantly, cone acetone extract (CA), cone ethanol extract (CE), and cone dichloromethane extract (CD) of Abies nordmanniana subsp. exhibited strong inhibition of the virus at a concentration of 10 µg/g. The most potent virucidal activity was observed with ethanol-water extract of conifer form (CEW). According to these results, it was proved that Abies nordmanniana species could be a potential, sustainable, and renewable drug source, especially considering the impressive antiviral and significant cytotoxic activity potentials.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Idioma: En Revista: Turk J Chem Año: 2024 Tipo del documento: Article

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Idioma: En Revista: Turk J Chem Año: 2024 Tipo del documento: Article