Human collagenase: identification and characterization of an enzyme from rheumatoid synovium in culture.
Science
; 158(3800): 499-502, 1967 Oct 27.
Article
en En
| MEDLINE
| ID: mdl-4292695
ABSTRACT
Synovial tissue from patients with rheumatoid arthritis produces lysis of gels of reconstituted collagen fibrils in culture and releases soluble collagenase when cultured in collagen-free medium. Collagen molecules in solution at neutral pH at 20 degrees and 27 degrees C are cleaved by the synovial enzyme into (3/4) and (1/4) length fragments. In this respect the action of synovial enzyme is similar to that of amphibian collagenase and distinct from that of bacterial collagenase. At 37 degrees C reconstituted collagen fibrils and native fibers are attacked by the enzyme and further degraded to polypeptides of low molecular weight. These polypeptides are produced only after denaturation of the larger fragments, which occurs at temperatures near 37 degrees C.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Artritis Reumatoide
/
Membrana Sinovial
/
Colagenasa Microbiana
Tipo de estudio:
Diagnostic_studies
Límite:
Humans
Idioma:
En
Revista:
Science
Año:
1967
Tipo del documento:
Article