A sandwich method of enzyme immunoassay. III. Assay for human beta-2 microglobulin compared with radioimmunoassay.
J Immunol Methods
; 36(2): 149-58, 1980.
Article
en En
| MEDLINE
| ID: mdl-6159424
ABSTRACT
A sandwich enzyme immunoassay has been developed to measure human beta-2 microglobulin (beta 2m) in bilogical fluids. beta 2m is first bound by specific antibody covalently coupled to microcrystalline cellulose. The solid phase is washed and reincubated with glucose oxidase-labeled anti-beta 2m antibody. After washing, the enzymic activity of the solid phase is measured by incubation with the appropriate chromogenic substrate. The OD is directly related to the quantity of beta 2m to be measured. A second sandwich assay has also been developed which uses plastic microplates coated with the IgG fraction of rabbit anti-beta 2m serum. Reproducible results are obtained in the range 2-150 and 0.75-48 microgram/l respectively. These tests detect 17 fmoles of beta 2m. Assays of 27 sera showed good agreement between these two enzyme immunoassay methods and two radioimmunoassays.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Beta-Globulinas
/
Radioinmunoensayo
/
Microglobulina beta-2
Límite:
Humans
Idioma:
En
Revista:
J Immunol Methods
Año:
1980
Tipo del documento:
Article