mRNA discrimination in extracts from uninfected and reovirus-infected L-cells.
J Virol
; 51(1): 215-22, 1984 Jul.
Article
en En
| MEDLINE
| ID: mdl-6328041
ABSTRACT
Uncapped reovirus mRNA extracted at late times from infected L-cells is preferentially translated in extracts from infected L-cells. However, translation of this uncapped, late, reovirus mRNA in extracts from infected cells is sensitive to inhibition by the cap analog m7GTP . These results imply that reovirus infection does not induce a transition from cap-dependent to cap-independent translation. Nevertheless, the results of in vitro translational competition experiments between L-cell mRNA and late viral mRNA were consistent with the view that reovirus does induce an alteration in the cap-dependent translational apparatus of L-cells. The reduced efficiency of translation of a variety of capped mRNAs in extracts from infected cells is also consistent with this notion. We further conclude that a factor exists in reovirus-infected L-cells that specifically stimulates translation of uncapped reovirus mRNAs.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Reoviridae
/
Orthoreovirus Mamífero 3
/
Biosíntesis de Proteínas
/
ARN Mensajero
/
ARN Viral
/
Infecciones por Reoviridae
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
J Virol
Año:
1984
Tipo del documento:
Article