Immunological and isoelectric focusing study of beta-glucocerebrosidase from normal and Gaucher disease.
Biochem Biophys Res Commun
; 120(2): 325-32, 1984 Apr 30.
Article
en En
| MEDLINE
| ID: mdl-6375656
ABSTRACT
Comparison of normal and Gaucher disease beta-glucocerebrosidase by agarose isoelectric focusing (IEF) demonstrated additional bands at the pI-6 area seen within the mutated enzyme, while both normal placenta and spleen enzyme preparations manifest only major activity at pI-5. Antiglucocerebrosidase antibodies precipitated both normal and pathological enzymes, however, more antibodies were needed to reach an equivalence with the normal enzymes than with the Gaucher's. Cross reactivity of the IEF isozymes were detected by direct immunodiffusion on the prefocused gel.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Enfermedad de Gaucher
/
Glucosidasas
/
Glucosilceramidasa
/
Isoenzimas
Límite:
Female
/
Humans
/
Pregnancy
Idioma:
En
Revista:
Biochem Biophys Res Commun
Año:
1984
Tipo del documento:
Article