Rapid assay of human plasma carboxypeptidase N by high-performance liquid chromatographic separation of hippuryl-lysine and its product.
J Chromatogr
; 266: 173-7, 1983 Aug 26.
Article
en En
| MEDLINE
| ID: mdl-6415078
ABSTRACT
A rapid and sensitive method for measuring carboxypeptidase N (CPN) activity in human plasma is described. The procedure is based on the hydrolysis of a high-specificity/low-affinity substrate, hippuryl-L-lysine, to its products hippuric acid and lysine. The substrate and product are separated quantitatively by high-performance liquid chromatography in less than 10 min following a minimal sample preparation time. The advantages of this method over previous ones are discussed and data are presented demonstrating the reliability of this method for the routine clinical determination of CPN activity.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Lisina Carboxipeptidasa
/
Carboxipeptidasas
/
Lisina
Límite:
Female
/
Humans
/
Male
Idioma:
En
Revista:
J Chromatogr
Año:
1983
Tipo del documento:
Article