A micro-method for the detection of butyrylcholinesterase secreted by hepatocytes in vitro.
Biosci Rep
; 4(2): 149-54, 1984 Feb.
Article
en En
| MEDLINE
| ID: mdl-6713085
ABSTRACT
A new assay has been developed for detection of butyrylcholinesterase (EC 3.1.1.8) activity based upon the change in absorbance of phenol red, caused by the release of butyric acid from the substrate. Using commercially available enzyme prepared from horse serum, linear, dose-related decreases in absorbance were obtained, generally with correlation values of 0.965 or greater. The assay was modified and used to detect enzyme activity in the supernatants from primary cultures of mouse hepatocytes. The enzyme-mediated response was inhibited by NN'-diisopropylphosphorodiamidic anhydride, a specific inhibitor of butyrylcholinesterase.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Butirilcolinesterasa
/
Colinesterasas
/
Hígado
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
Idioma:
En
Revista:
Biosci Rep
Año:
1984
Tipo del documento:
Article