Analysis of time-resolved fluorescence anisotropy decays.
Biophys J
; 46(1): 45-56, 1984 Jul.
Article
en En
| MEDLINE
| ID: mdl-6743756
ABSTRACT
We discuss the analysis of time-correlated single photon counting measurements of fluorescence anisotropy. Particular attention was paid to the statistical properties of the data. The methods used previously to analyze these experiments were examined and a new method was proposed in which parallel- and perpendicular-polarized fluorescence curves were fit simultaneously. The new method takes full advantage of the statistical properties of the measured curves; and, in some cases, it is shown to be more sensitive than other methods to systematic errors present in the data. Examples were presented using experimental and simulated data. The influence of fitting range on extracted parameters and statistical criteria for evaluating the quality of fits are also discussed.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Polarización de Fluorescencia
/
Modelos Químicos
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
Biophys J
Año:
1984
Tipo del documento:
Article