[Fractionation of the cellulase complex of the fungus Aspergillus terreus]. / Fraktsionirovanie tselliulaznogo kompleksa griba Aspergillus terreus.

ABSTRACT

A procedure for isolating a complex of extracellular cellulases from the Aspergillus terreus culture liquid using SP-Sephadex C-50 has been developed. Enzymatically and electrophoretically homogeneous preparations of cellobiase I and cellobiase II have been obtained. By means of gel-filtration their molecular weights have been estimated to be 140,000 and 67,000. Cellobiase I consists of five and cellobiase II of two polypeptide chains. Three cellulases with various ratios of endoglucanase and exoglucosidase activities have been prepared. Using polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate, their molecular weights have been found to be 11,600; 10,900 and 11,200.