Detection and quantification of the organophosphate insecticide paraoxon by competitive inhibition enzyme immunoassay.
Life Sci
; 30(4): 355-61, 1982 Jan 25.
Article
en En
| MEDLINE
| ID: mdl-7040891
ABSTRACT
A competitive inhibition enzyme immunoassay (EIA) was developed to detect and quantify levels of the organophosphate insecticide paraoxon in body fluids. Protein-conjugated paraoxon served as an immunogen for the production of rabbit heteroantiserum, from which affinity purified IgG anti-paraoxon antibodies were isolated using a heterologous protein-paraoxon-conjugated immunoabsorbent. In the competitive inhibition EIA a standard curve was generated for the inhibition of binding of anti-paraoxon IgG to a solid-phase bound heterologous protein-paraoxon conjugate by various concentrations of free paraoxon. Binding was proportionate to the color change of an appropriate substrate generated by an enzyme-conjugated second antibody specific for the rabbit IgG anti-paraoxon. The assay detected paraoxon levels as low as 10(-10)M (28 pg/ml) in buffer, and serum paraoxon levels as low as 10(-9)M. In addition to its sensitivity, this technique is ideally suited to the simultaneous processing of large numbers of samples in less than 2 hr. The competitive inhibition EIA is cost effective and should facilitate environmental surveillance using sentinel animals, expand laboratory toxicology studies, and improve clinical detection capabilities.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Paraoxon
/
Técnicas para Inmunoenzimas
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
/
Female
/
Humans
/
Male
Idioma:
En
Revista:
Life Sci
Año:
1982
Tipo del documento:
Article