Identification of nucleolus organizer regions (NORs) in normal and neoplastic human cells by the silver-staining technique.
Cytogenet Cell Genet
; 19(4): 185-96, 1977.
Article
en En
| MEDLINE
| ID: mdl-74306
ABSTRACT
Silver nitrate has been used to demonstrate the chromosomal location of ribosomal cistrons in nine tissue-culture lines derived from human tumors of various pathological origins. Control individuals have a particular modal number (range 7--10) of D- and G-group chromosomes stained with silver. In the controls, 96.2% of the D- and G-group chromosomes that have a stalk show silver staining, while no relationship can be seen in acrocentric chromosomes without stalks. The tumor cells, whose modal chromosome numbers range from 42 to 68, possess variable numbers of acrocentrics (11--18). The number of chromosomes stained with silver, however, remained at control levels (range, 6--9). These data indicate that, in humans, silver staining may not identify all NORs that contain structural ribosomal genes.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Nitrato de Plata
/
Nucléolo Celular
/
Neoplasias
Tipo de estudio:
Diagnostic_studies
/
Prognostic_studies
Límite:
Female
/
Humans
Idioma:
En
Revista:
Cytogenet Cell Genet
Año:
1977
Tipo del documento:
Article