Stepdown hyperthermia in human melanoma cells: effects on protracted mild hyperthermia for survival and DNA polymerase inactivation.
Melanoma Res
; 5(4): 229-34, 1995 Aug.
Article
en En
| MEDLINE
| ID: mdl-7496157
ABSTRACT
Human melanoma cells have been used to evaluate whether stepdown heating (SDH) could increase the effectiveness of long-duration mild hyperthermia (LDMH). The effects of these treatments were also evaluated on cell survival and DNA polymerase inactivation. Short treatments (30 min) at 43 degrees C did not result in much SDH effect for subsequent protracted heating at 40 degrees C. The effect on DNA polymerases was also very small. However, heating at 44 degrees C for 30 min had a large SDH heating effect on subsequent heating at 40 degrees C and 41 degrees C. The SDH effect occurred mainly in the first 5-10 h of subsequent LDMH and, at longer heating times, the rate of cell killing was reduced. The 44 degrees C SDH effect was also observed on DNA polymerase inactivation. Comparing the degree of cell killing and polymerase inactivation showed a good correlation for the various SDH protocols.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
ADN Polimerasa Dirigida por ADN
/
Hipertermia Inducida
/
Melanoma
Tipo de estudio:
Diagnostic_studies
/
Evaluation_studies
/
Guideline
Límite:
Humans
Idioma:
En
Revista:
Melanoma Res
Asunto de la revista:
NEOPLASIAS
Año:
1995
Tipo del documento:
Article
País de afiliación:
Canadá