The nad6 gene and the exon d of nad1 are co-transcribed in wheat mitochondria.
Curr Genet
; 24(6): 533-8, 1993 Dec.
Article
en En
| MEDLINE
| ID: mdl-7507801
ABSTRACT
The exon d of nad1 is located 993 bp upstream of the nad6 gene in the wheat mitochondrial genome. Transcription analyses of both sequences (nad1 exon d and the nad6 gene) were done by Northern hybridization using RNA from wheat seedlings and tissue cultures derived from immature embryos. A complicated pattern was generated with a probe including exon d of nad1 and the whole nad6 gene. An 0.71-kb transcript is specific to nad1 exon d whereas a 1.2-kb transcript is specific to the nad6 gene. Three larger transcripts hybridize to both probes suggesting that nad1 exon d and nad6 are co-transcribed. This co-transcription has been directly demonstrated by cDNA synthesis on mtRNAs and sequencing of the PCR amplification product.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Triticum
/
Exones
/
NAD(P)H Deshidrogenasa (Quinona)
/
Genes de Plantas
/
Mitocondrias
Idioma:
En
Revista:
Curr Genet
Año:
1993
Tipo del documento:
Article
País de afiliación:
Francia