Spectroscopic evidence for an intramolecular RNA triple helix.
FEBS Lett
; 355(3): 297-300, 1994 Dec 05.
Article
en En
| MEDLINE
| ID: mdl-7527349
ABSTRACT
A 29-base RNA oligomer has been chemically synthesized and shown to form an intramolecular triple helix in solution at acidic pH. Assignment of the majority of the exchangeable proton NMR resonances demonstrated the Watson-Crick and Hoogsteen base pairings consistent with folding to form pyrimidine-purine-pyrimidine base triplets. FTIR spectroscopy provided independent evidence of base triplet formation, and indicated a predominately C3'-endo sugar pucker. UV absorption as a function of temperature suggested monophasic melting behaviour, which was confirmed by NMR of the imino protons.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
ARN
/
Conformación de Ácido Nucleico
Idioma:
En
Revista:
FEBS Lett
Año:
1994
Tipo del documento:
Article
País de afiliación:
Francia