A paternal-specific methylation imprint marks the alleles of the mouse H19 gene.
Nat Genet
; 9(4): 407-13, 1995 Apr.
Article
en En
| MEDLINE
| ID: mdl-7795647
ABSTRACT
Imprinting, the differential expression of the two alleles of a gene based on their parental origin, requires that the alleles be distinguished or marked. A candidate for the differentiating mark is DNA methylation. The maternally expressed H19 gene is hypermethylated on the inactive paternal allele in somatic tissues and sperm, but to serve as the mark that designates the imprint, differential methylation must also be present in the gametes and the pre-implantation embryo. We now show that the pattern of differential methylation in the 5' portion of H19 is established in the gametes and a subset is maintained in the pre-implantation embryo. That subset is sufficient to confer monoallelic expression to the gene in blastocysts. We propose that paternal-specific methylation of the far 5' region is the mark that distinguishes the two alleles of H19.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Paternidad
/
ADN
/
Impresión Genómica
/
Alelos
Límite:
Animals
Idioma:
En
Revista:
Nat Genet
Asunto de la revista:
GENETICA MEDICA
Año:
1995
Tipo del documento:
Article
País de afiliación:
Estados Unidos