Cloning and expression of a cDNA for rat prostacyclin receptor.
Biochim Biophys Acta
; 1224(3): 601-5, 1994 Dec 30.
Article
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| MEDLINE
| ID: mdl-7803522
ABSTRACT
A cDNA clone for rat prostacyclin receptor was isolated. The cDNA encodes a protein of 416 amino acid residues (M(r) 44,662) with putative seven transmembrane domains, and belongs to the G protein-coupled receptor superfamily. Specific binding of [3H]iloprost was found in membrane of COS-7 cells transfected with the cDNA (Kd = 1.3 nM) and was displaced with unlabeled prostaglandins in the order of iloprost = cicaprost > PGE1 > STA2 = PGE2 = PGD2 > PGF2 alpha. Northern blot analysis demonstrated that rat prostacyclin receptor mRNA is expressed in the lung, spleen, heart, pancreas, thymus, stomach and aorta.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Receptores de Prostaglandina
Límite:
Animals
Idioma:
En
Revista:
Biochim Biophys Acta
Año:
1994
Tipo del documento:
Article
País de afiliación:
Japón