Heterologous biosynthesis and processing of preprovasopressin in Neuro2A neuroblastoma cells.
Biochimie
; 76(3-4): 315-9, 1994.
Article
en En
| MEDLINE
| ID: mdl-7819342
ABSTRACT
To obtain a model for the sorting and processing of preprovasopressin (preproVP), rat VP cDNA was transfected in murine Neuro2A neuroblastoma cells, which do not express VP. The precursor of VP was expressed and processed into the authentic VP gene products VP, neurophysin (NP) and glycopeptide (GP) as determined with reversed phase HPLC and radioimmunoassay. In addition, Neuro2A-specific forms of NP and GP were observed, which may be produced in the constitutive secretory pathway in these cells.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Hipófisis
/
Precursores de Proteínas
/
Vasopresinas
/
Expresión Génica
/
Procesamiento Proteico-Postraduccional
/
Hipotálamo
Límite:
Animals
Idioma:
En
Revista:
Biochimie
Año:
1994
Tipo del documento:
Article
País de afiliación:
Países Bajos