Dissecting the centromere of the human Y chromosome with cloned telomeric DNA.
Hum Mol Genet
; 3(8): 1227-37, 1994 Aug.
Article
en En
| MEDLINE
| ID: mdl-7987296
ABSTRACT
We have used telomeric DNA to break the human Y chromosome within the centromeric array of alphoid satellite DNA and have created two derivative chromosomes; one consists of the short arm and 140 kb of alphoid DNA, the other consists of the long arm and 480 kb of alphoid DNA. Both segregate accurately at mitosis. It is known that there is no large scale sequence duplication around the alphoid DNA and so the simplest interpretation of our results is that the sequence responsible for accurate segregation is the alphoid DNA itself. Although the long arm acrocentric derivative segregates accurately it lags with respect to the other chromosomes in about 10% of anaphase cells and thus additional sequences may be required for orderly segregation. The short arm acrocentric chromosome is probably no larger than 12 Mb in size and thus our results also demonstrate that chromosomes of this size are capable of accurate segregation.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Cromosoma Y
/
ADN
/
Centrómero
/
Clonación Molecular
Límite:
Humans
Idioma:
En
Revista:
Hum Mol Genet
Asunto de la revista:
BIOLOGIA MOLECULAR
/
GENETICA MEDICA
Año:
1994
Tipo del documento:
Article
País de afiliación:
Reino Unido