An immunoperoxidase plaque assay for reticuloendotheliosis virus and its application to a sensitive serum neutralization assay.
Avian Dis
; 38(1): 165-71, 1994.
Article
en En
| MEDLINE
| ID: mdl-8002888
ABSTRACT
A rapid assay for the enumeration of reticuloendotheliosis virus (REV) is described. Chicken embryo fibroblast monolayer cultures were infected with REV and incubated 6 days under an agar overlay. After removal of the overlay, cells were fixed with acetone/ethanol. Foci of infection (hereafter referred to as plaques) were detected using either an anti-REV envelope monoclonal antibody or convalescent chicken serum as the primary antibody; the secondary antibody was either horseradish peroxidase-conjugated goat anti-mouse IgG (for use with monoclonals) or goat anti-chicken IgG (for use with chicken serum). Staining with a substrate solution containing diaminobenzidine, CoCl2, and H2O2 revealed individual dark plaques on a light gray background. This method worked equally well for the SNV, CSV, and REV-T strains of REV; further, it detected all six field isolates tested. Results indicate that this immunoperoxidase technique is a rapid and reliable method for detection and titration of REV as well as for the assay of neutralizing antibody in chicken serum.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Enfermedades de las Aves de Corral
/
Infecciones Tumorales por Virus
/
Pruebas de Neutralización
/
Virus de la Reticuloendoteliosis
/
Infecciones por Retroviridae
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
Idioma:
En
Revista:
Avian Dis
Año:
1994
Tipo del documento:
Article