The predicted amino acid sequence of human thioredoxin is identical to that of the autocrine growth factor human adult T-cell derived factor (ADF): thioredoxin mRNA is elevated in some human tumors.
Biochim Biophys Acta
; 1218(3): 292-6, 1994 Aug 02.
Article
en En
| MEDLINE
| ID: mdl-8049254
ABSTRACT
The cDNA sequences of thioredoxin obtained by PCR cloning from human colon cancer cells, human lymphoblastoid cells, and human liver have been found to be identical with the cDNA sequence reported for the autocrine growth factor, human adult T-cell leukemia derived factor (ADF). Recombinant human thioredoxin was 95% reduced by dithiothreitol and was a substrate for reduction by human thioredoxin reductase. Human non-small cell primary lung tumors from subjects who were not cigarette smokers at the time of surgery showed significantly increased levels of thioredoxin mRNA compared to thioredoxin mRNA in paired normal human lung tissue. Subjects who were smokers did not show a significant increase in lung tumor thioredoxin mRNA. The results of the study show that human thioredoxin and ADF are identical species and suggest that there may be increased production of thioredoxin (ADF) by some human cancers.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Tiorredoxinas
/
ARN Mensajero
/
Expresión Génica
/
Citocinas
/
Proteínas de Neoplasias
Tipo de estudio:
Prognostic_studies
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Risk_factors_studies
Límite:
Adult
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Animals
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Humans
Idioma:
En
Revista:
Biochim Biophys Acta
Año:
1994
Tipo del documento:
Article