A new ATP-independent DNA endonuclease from Schizosaccharomyces pombe that recognizes cyclobutane pyrimidine dimers and 6-4 photoproducts.

Bowman, K K; Sidik, K; Smith, C A; Taylor, J S; Doetsch, P W; Freyer, G A

Bowman, K K; Sidik, K; Smith, C A; Taylor, J S; Doetsch, P W; Freyer, G A.

Afiliación

Bowman KK; Department of Biochemistry, Emory University School of Medicine, Rollins Research Center, Atlanta, GA 30322.

Nucleic Acids Res ; 22(15): 3026-32, 1994 Aug 11.

Article en En | MEDLINE | ID: mdl-8065916

ABSTRACT

ABSTRACT

We have discovered a new DNA endonuclease in the fission yeast Schizosaccharomyces pombe which recognizes cyclobutane pyrimidine dimers and (6-4) pyrimidine-pyrimidone photoproducts. S. pombe DNA endonuclease (SPDE) catalyzes a single ATP-independent incision immediately 5' to the UV photoproduct and generates termini containing 3' hydroxyl and 5' phosphoryl groups. Based on these properties, we propose that SPDE may function in a DNA repair capacity, representing the initial recognition/cleavage step of a DNA excision repair pathway.

Asunto(s)

Adenosina Trifosfato/farmacología; Reparación del ADN; Endodesoxirribonucleasas/metabolismo; Dímeros de Pirimidina/metabolismo; Proteínas de Schizosaccharomyces pombe; Schizosaccharomyces/enzimología; Secuencia de Bases; ADN/química; ADN/metabolismo; Daño del ADN; Datos de Secuencia Molecular; Fotoquímica; Especificidad por Sustrato; Rayos Ultravioleta

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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Dímeros de Pirimidina / Schizosaccharomyces / Adenosina Trifosfato / Proteínas de Schizosaccharomyces pombe / Reparación del ADN / Endodesoxirribonucleasas Idioma: En Revista: Nucleic Acids Res Año: 1994 Tipo del documento: Article

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