Telomere-associated chromosome breakage in fission yeast results in variegated expression of adjacent genes.
EMBO J
; 13(16): 3801-11, 1994 Aug 15.
Article
en En
| MEDLINE
| ID: mdl-8070408
ABSTRACT
The sequence requirements for in vivo telomere function in the fission yeast, Schizosaccharomyces pombe, have been investigated. A 258 bp tract of previously characterized cloned fission yeast terminal repeats adjacent to 800 bp of telomere-associated sequences is sufficient to seed new telomeres onto linearized ars-containing plasmids when introduced into cells. The resulting transformants contain unrearranged, acentric, linear episomes. Cloned telomeres, with and without telomere-associated sequences adjacent to the 258 bp terminal repeats, were utilized to introduce chromosome breaks at specific sites in a non-essential minichromosome. Truncated minichromosome derivatives were recovered containing the ura4 or ade6 gene adjacent to a newly formed telomere. These telomeres exert reversible position effects on the expression of the adjacent ura4 or ade6 genes.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Schizosaccharomyces
/
Regulación Fúngica de la Expresión Génica
/
Aberraciones Cromosómicas
/
Genes Fúngicos
Tipo de estudio:
Risk_factors_studies
Idioma:
En
Revista:
EMBO J
Año:
1994
Tipo del documento:
Article
País de afiliación:
Reino Unido