Correlation between E2F-1 requirement in the S phase and E2F-1 transactivation of cell cycle-related genes in human cells.
Cancer Res
; 54(6): 1402-6, 1994 Mar 15.
Article
en En
| MEDLINE
| ID: mdl-8137237
ABSTRACT
The mammalian nuclear protein E2F-1 has recently been cloned based on its ability to bind the retinoblastoma protein. To determine whether E2F-1 plays a role in the control of the cell proliferation, we introduced an inducible construct expressing an E2F-1 antisense RNA into the human glioblastoma T98G cell line and assessed DNA synthesis during the cell cycle. Expression of the antisense transcripts during the G1-S transition resulted in a marked delay in the completion of DNA synthesis. Band-shift analysis of bacterially produced E2F-1 showed that this protein bound to the promoters of human DNA polymerase-alpha, cyclin D1, and c-myb but not to the cdc2 gene promoter. E2F-1 also transactivated the bound promoters in transient transfection assays. These results suggest a major role for E2F-1 in the control of cell cycle progression via transcriptional regulation of proliferation-associated genes.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Factores de Transcripción
/
Transcripción Genética
/
Proteínas Portadoras
/
Activación Transcripcional
/
Fase S
/
Regiones Promotoras Genéticas
/
Proteínas de Ciclo Celular
/
Proteínas de Unión al ADN
Límite:
Humans
Idioma:
En
Revista:
Cancer Res
Año:
1994
Tipo del documento:
Article