Single crystals of a type III antifreeze polypeptide from ocean pout.
J Mol Biol
; 237(3): 351-2, 1994 Apr 01.
Article
en En
| MEDLINE
| ID: mdl-8145247
ABSTRACT
Antifreeze protein (HPLC-2) from ocean pout was purified from serum using column chromatography on Sephadex G75 and reverse-phase HPLC columns. Single crystals were grown by batch methods at 4 degrees C from a 1.5 M solution of ammonium sulphate (pH 7.1). The crystals diffracted to about 2.5 A resolution at 4 degrees C and belong to the monoclinic space group P2(1), with cell parameters a = 39.77 A, b = 58.51 A, c = 30.27 A, beta = 102.28 degrees, with two molecules of 6000 M(r) per asymmetric unit.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Glicoproteínas
/
Peces
Límite:
Animals
Idioma:
En
Revista:
J Mol Biol
Año:
1994
Tipo del documento:
Article
País de afiliación:
Canadá