Antirheumatic gold compounds and penicillamine enhance protein kinase C-mediated activation of the arachidonate-mobilizing phospholipase A2 in mouse macrophages.
J Leukoc Biol
; 54(4): 329-35, 1993 Oct.
Article
en En
| MEDLINE
| ID: mdl-8409755
The effects of antirheumatic gold compounds and D-penicillamine on protein kinase C- and Ca(2+)-mediated activation of arachidonate mobilization and the formation of eicosanoids in mouse macrophages have been investigated. Auranofin (0.2-2 microM) enhanced the response to phorbol ester two- to three-fold, and similar enhancement was caused by aurothiomalate, aurothioglucose, and penicillamine, but only after pretreatment for 1-4 h. The enhanced mobilization of arachidonate was accompanied by increased formation and release of prostaglandin E2 and 6-keto prostaglandin F1 alpha, but not of lipoxygenase metabolites. No such enhancement occurred when the arachidonate-mobilizing phospholipase A2 was activated directly (calcium ionophore A23187). Instead, auranofin caused selective inhibition of calcium ionophore-induced formation of leukotriene C4. Treatment of macrophages with 4 beta-phorbol 12-myristate 13-acetate causes a rapid increase in the phosphorylation and a 1.4-1.8-fold increase in the activity of the 85-kd arachidonate-mobilizing phospholipase A2 as determined in an in vitro assay. The increase in activity was further enhanced by both the gold compounds and penicillamine. These findings indicate that the target for the enhancing effect of the antirheumatic drugs is located between protein kinase C and phospholipase A2 in the signal chain leading to activation of the latter enzyme.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Penicilamina
/
Fosfolipasas A
/
Proteínas Quinasas
/
Eicosanoides
/
Aurotioglucosa
/
Auranofina
/
Ácido Araquidónico
/
Tiomalato Sódico de Oro
/
Macrófagos Peritoneales
Límite:
Animals
Idioma:
En
Revista:
J Leukoc Biol
Año:
1993
Tipo del documento:
Article
País de afiliación:
Suecia