A simple reliable system for studying antigen-specific murine T cell proliferation.
J Immunol Methods
; 25(2): 159-70, 1979.
Article
en En
| MEDLINE
| ID: mdl-84843
ABSTRACT
Antigen-specific T cell proliferation can be readily elicited from the popliteal lymph node cells of mice which have received immunizations of antigen in the hind footpads. The advantages of our system over other published methods are (i) simplicity in method and materials, (ii) much improved reproducibility, (iii) negligible concomitant B cell proliferation, (iv) large degrees of antigen specific proliferation with very low background, and (v) complete dependence of the response on accessory cells or macrophages. These results were brought about by proper immunization procedures for mice and judicious choice of culture conditions. Our data show that the system is very suitable for the study of macrophage-T cell interaction in the induction of T cell proliferation as well as the genetic basis of responsiveness or non-responsiveness to protein and polypeptide antigens.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Linfocitos T
Límite:
Animals
Idioma:
En
Revista:
J Immunol Methods
Año:
1979
Tipo del documento:
Article