Human Philadelphia chromosome-positive chronic myeloid leukemia: a potential model for antisense therapy.
Exp Hematol
; 21(6): 826-8, 1993 Jun.
Article
en En
| MEDLINE
| ID: mdl-8500581
ABSTRACT
We have developed an in vivo model of human chronic myeloid leukemia (CML). A peripheral blood (PB) sample of Philadelphia (Ph) chromosome-positive CML cells in lymphoid blast crisis was transplanted intravenously (IV) into sublethally irradiated severe combined immunodeficient (SCID) mice, and this resulted in engraftment with systemic proliferation. Growth of leukemia was monitored by PB cell morphology and by flow cytometric analysis of murine PB cells labelled with an anti-human leukocyte antigen (HLA) monoclonal antibody. Human cells were first detected in the PB at 4 weeks and comprised a mean of 57% of the total nucleated cells in the PB of these mice by 15 weeks. The Ph chromosome was retained and the population has been successfully passaged. BCR/ABL fusion gene expression was detected in a subsequent passage. Experiments are underway to use this in vivo model to assess the antileukemic activity of BCR/ABL antisense oligonucleotides.
Buscar en Google
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Proteínas Tirosina Quinasas
/
Leucemia Mielógena Crónica BCR-ABL Positiva
/
Oligonucleótidos Antisentido
Límite:
Animals
/
Female
/
Humans
Idioma:
En
Revista:
Exp Hematol
Año:
1993
Tipo del documento:
Article
País de afiliación:
Reino Unido