The interaction of adriamycin and its beta anomer with DNA.
Biochim Biophys Acta
; 476(1): 38-46, 1977 May 03.
Article
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| MEDLINE
| ID: mdl-856282
ABSTRACT
The results of thermal denaturation, fluorescence, calorimetric and viscosimetric studies on the interaction of adriamycin and its beta anomer with DNA are reported. Whereas all equilibrium, hydrodynamic and thermodynamic measurements are consistent with the proposed intercalative binding model for the adriamycin-DNA complex, the binding mechanism for the reaction of the beta anomer with DNA remains uncertaian. All DNA binding properties of this stereoisomer are substantially different from those of the parent compound. The results suggest that the amino sugar residue of the natural antibiotic may interact stero-specifically with the DNA helix, thus dictating the orientation of the tetracvclic chromophore within the intercalation site. The alteration in the DNA binding capacity and the changes in interactions with DNA following in inversion of configuration at C-1', parallel a lack of biological activity observed for the beta anomer.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
ADN
/
Doxorrubicina
Idioma:
En
Revista:
Biochim Biophys Acta
Año:
1977
Tipo del documento:
Article