TPA and cycloheximide modulate the activation of NF-kappa B and the induction and stability of nitric oxide synthase transcript in primary neonatal rat hepatocytes.
FEBS Lett
; 379(3): 279-85, 1996 Feb 05.
Article
en En
| MEDLINE
| ID: mdl-8603706
ABSTRACT
12-O-Tetradecanoylphorbol 13-acetate (TPA) elicited a transient increase in the transcription of the inducible nitric oxide synthase (iNOS) gene coupled with a shortening of the half-life of its mRNA in primary neonatal rat hepatocytes. These effects of TPA were preceded by a surge in nuclear translocation of the transcription factor NF-kappa B, and followed by a mounting accumulation of NO-2 in the growth medium. Even cycloheximide (CHX) added by itself elicited an early, sustained activation of NF-kappa B followed by an intense induction of iNOS gene expression, irrespective of what degree of protein synthesis inhibition was brought about by the several concentrations tested. When given together, TPA and CHX exerted additive effects on hepatocellular iNOS mRNA levels. These results suggest the likelihood of an ordered sequence of events by which an activated NF-kappa B mediates the induction of iNOS gene expression in TPA- and/or CHX-treated primary hepatocytes.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Acetato de Tetradecanoilforbol
/
FN-kappa B
/
Óxido Nítrico Sintasa
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Cicloheximida
/
Hígado
Límite:
Animals
Idioma:
En
Revista:
FEBS Lett
Año:
1996
Tipo del documento:
Article
País de afiliación:
Italia