A role for the Ral guanine nucleotide dissociation stimulator in mediating Ras-induced transformation.
J Biol Chem
; 271(28): 16439-42, 1996 Jul 12.
Article
en En
| MEDLINE
| ID: mdl-8663585
Oncogenic Ras transforms cells through the activation of multiple downstream pathways mediated by separate effector molecules, one of which is Raf. Here we report the identification of a second ras-binding protein that can induce cellular transformation in parallel with activation of the Raf/mitogen-activated protein kinase cascade. The Ral guanine nucleotide dissociation stimulator (RalGDS) was isolated from a screen for Ras-binding proteins that specifically interact with a Ras effector-loop mutant, ras(12V,37G), that uncouples Ras from activation of Raf1. RalGDS, like ras(12V, 37G), cooperates synergistically with mutationally activated Raf to induce foci of growth and morphologically transformed NIH 3T3 cells. RalGDS does not significantly enhance MAP kinase activation by activated Raf, suggesting that the cooperativity in focus formation is due to a distinct pathway acting downstream of Ras and parallel to Raf.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Proteína Oncogénica p21(ras)
/
Transformación Celular Neoplásica
/
Proteínas de Unión al GTP
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
J Biol Chem
Año:
1996
Tipo del documento:
Article
País de afiliación:
Estados Unidos