Antibodies to p24 antigen do not specifically detect HIV-infected lymphocytes in AIDS patients.
J Immunol Methods
; 191(2): 179-86, 1996 May 27.
Article
en En
| MEDLINE
| ID: mdl-8666837
ABSTRACT
A flow cytometric assay (FCA), which detects the p24 antigen in HIV-infected cell lines and in peripheral blood mononuclear cells (PBMC) of AIDS patients, has been described in several studies. However, the results presented here clearly show that this p24-FCA, although useful for the analysis of HIV infection of cells in vitro, does not specifically detect HIV-infected PBMC from patients. Isotype control antibodies also stained PBMC from HIV-infected patients to a greater degree than the PBMC from healthy controls. Furthermore, the CD4-negative lymphocytes, which are generally not infected with HIV, were also found to stain with anti-p24. Finally, no enrichment of HIV-infected cells was found in the FACS-purified CD4+p24+ lymphocytes, compared to the CD4+p24- cell fraction. The p24-FCA, therefore, was not useful for determining the percentage of infected PBMCs from HIV-infected individuals.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Anticuerpos Anti-VIH
/
Linfocitos T CD4-Positivos
/
Síndrome de Inmunodeficiencia Adquirida
/
Proteína p24 del Núcleo del VIH
/
Especificidad de Anticuerpos
Límite:
Humans
Idioma:
En
Revista:
J Immunol Methods
Año:
1996
Tipo del documento:
Article
País de afiliación:
Alemania