Analysis of cathepsin D from breast tissues by capillary electrophoresis.
J Chromatogr B Biomed Appl
; 683(1): 125-31, 1996 Aug 09.
Article
en En
| MEDLINE
| ID: mdl-8876448
ABSTRACT
A rapid and simple method for analyzing cathepsin D in breast tissue based on capillary zone electrophoresis (CZE) is described. After incubating the tissue extracts with hemoglobin as a substrate, a specific peptide is cleaved and separated by CZE in less than 5 min. This peptide is not produced by the action of pepsin or trypsin. It is inhibited by the addition of pepstatin, a specific inhibitor for cathepsin D. Human hemoglobin acted as a better substrate than bovine hemoglobin. The test compared well to a radioimmunoassay. We have shown that peptides can be stacked by the use of acetonitrile. The method demonstrates the advantages of CZE for assay of proteolytic enzymes in general.
Buscar en Google
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Mama
/
Catepsina D
/
Electroforesis Capilar
Tipo de estudio:
Diagnostic_studies
Límite:
Humans
Idioma:
En
Revista:
J Chromatogr B Biomed Appl
Año:
1996
Tipo del documento:
Article
País de afiliación:
Estados Unidos