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Dependence of growth, metabolic expression, and pathogenicity of Naegleria fowleri on exogenous porphyrins.
Bradley, S G; Toney, D M; Zhang, Y; Marciano-Cabral, F.
Afiliación
  • Bradley SG; Department of Microbiology and Immunology, Virginia Commonwealth University, Richmond 23298-0678, USA.
J Parasitol ; 82(5): 763-8, 1996 Oct.
Article en En | MEDLINE | ID: mdl-8885886
The pathogenicity of the free-living amoeba Naegleria fowleri is modulated by the composition of the medium used for cultivation. The constituents that determine the level of pathogenicity of N. fowleri, however, have not been definitively established. The present study examined the effects of selected porphyrins on N. fowleri amoebae. The iron-containing porphyrins, hemin or hematin, or the iron-free porphyrin, protoporphyrin IX, were effective in supporting growth of N. fowleri in Cline medium lacking serum. Iron-binding proteins, including hemoglobin, could not satisfy the growth requirement of the amoebae for exogenous porphyrin. Expression of biological functions including azocaseinase activity, agglutination, mobility, complement susceptibility, and virulence were altered by the composition of the growth medium. Amoebae grown in Cline medium supplemented with either hemin or protoporphyrin IX displayed greater mobility and were more resistant to lysis by complement than those grown in Nelson medium. Similarly, amoebae grown in Cline medium supplemented with either hemin or protoporphyrin IX were more pathogenic for B6C3F1 mice than those grown in Nelson medium. The addition of protoporphyrin IX to Nelson medium resulted in a modest increase in mobility, resistance to complement lysis and virulence when compared to N. fowleri amoebae grown in Nelson medium without added porphyrin.
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Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Porfirinas / Naegleria fowleri / Amebiasis Límite: Animals Idioma: En Revista: J Parasitol Año: 1996 Tipo del documento: Article País de afiliación: Estados Unidos
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Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Porfirinas / Naegleria fowleri / Amebiasis Límite: Animals Idioma: En Revista: J Parasitol Año: 1996 Tipo del documento: Article País de afiliación: Estados Unidos