Construction of vectors and a genomic library for use with his3-deficient strains of Schizosaccharomyces pombe.
Gene
; 174(2): 315-8, 1996 Oct 03.
Article
en En
| MEDLINE
| ID: mdl-8890754
ABSTRACT
The construction of vectors for use in Schizosaccharomyces pombe using the his3+ gene as a selectable marker is described. In addition, we report the construction of a genomic library in a his3(+)-containing shuttle vector to facilitate the cloning of genes by complementation of mutant function in strains defective for His3 activity.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Schizosaccharomyces
/
Biblioteca de Genes
/
Vectores Genéticos
/
Hidroliasas
Idioma:
En
Revista:
Gene
Año:
1996
Tipo del documento:
Article
País de afiliación:
Estados Unidos