Construction of vectors and a genomic library for use with his3-deficient strains of Schizosaccharomyces pombe.

Ohi, R; Feoktistova, A; Gould, K L

Ohi, R; Feoktistova, A; Gould, K L.

Afiliación

Ohi R; Howard Hughes Medical Institute, Vanderbilt University, School of Medicine, Nashville, TN 37232, USA.

Gene ; 174(2): 315-8, 1996 Oct 03.

Article en En | MEDLINE | ID: mdl-8890754

ABSTRACT

ABSTRACT

The construction of vectors for use in Schizosaccharomyces pombe using the his3+ gene as a selectable marker is described. In addition, we report the construction of a genomic library in a his3(+)-containing shuttle vector to facilitate the cloning of genes by complementation of mutant function in strains defective for His3 activity.

Asunto(s)

Biblioteca de Genes; Vectores Genéticos/genética; Hidroliasas/genética; Schizosaccharomyces/genética; Clonación Molecular; ADN Recombinante; Prueba de Complementación Genética; Marcadores Genéticos; Variación Genética; Plásmidos

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Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Schizosaccharomyces / Biblioteca de Genes / Vectores Genéticos / Hidroliasas Idioma: En Revista: Gene Año: 1996 Tipo del documento: Article País de afiliación: Estados Unidos

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