Correlation of ATP/citrate lyase activity with lipid accumulation in developing seeds of Brassica napus L.
Lipids
; 32(1): 7-12, 1997 Jan.
Article
en En
| MEDLINE
| ID: mdl-9075187
ABSTRACT
The temporal distribution of ATP/citrate lyase (ACL) activity in developing seeds of Brassica napus L. closely paralleled both that of acetyl-CoA carboxylase and the overall rate of lipid biosynthesis. Maximum ACL activities (250 nmol acetyl-CoA formed min-1.g fresh seed) were recorded between 35 to 42 d after pollination and, if the in vitro data could be extrapolated to the situation in vivo, could account for half of the acetyl-CoA required for the measured rate of fatty acid biosynthesis during seed development. The enzyme appeared to be localized in a subcellular compartment, which was clearly separated from mitochondria on a sucrose gradient and by differential centrifugation, and which corresponded to the chloroplast organelle.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
ATP Citrato (pro-S)-Liasa
/
Brassica
/
Metabolismo de los Lípidos
Idioma:
En
Revista:
Lipids
Año:
1997
Tipo del documento:
Article
País de afiliación:
Reino Unido