A dominant mutation in the Chlamydomonas reinhardtii nuclear gene SIM30 suppresses translational defects caused by initiation codon mutations in chloroplast genes.
Genetics
; 145(4): 935-43, 1997 Apr.
Article
en En
| MEDLINE
| ID: mdl-9093848
ABSTRACT
A suppressor of a translation initiation defect caused by an AUG to AUU mutation in the Chlamydomonas reinhardtii chloroplast petD gene was isolated, defining a nuclear locus that we have named SIM30. A dominant mutant allele at this locus, sim30-1d, was found to increase the translation initiation rate of the mutant petD mRNA. sim30-1d was also able to suppress the translational defect caused by an AUG to AUC mutation in the petD gene, and an AUG to AUU mutation in the chloroplast petA gene. We therefore suggest that the SIM30 gene may encode a general chloroplast translation factor. The ability of sim30-1d to suppress the petD AUG to AUU mutation is diminished in the presence of one or more antibiotic resistance markers located within the 16S and 23S rRNAs, suggesting that the activity of the sim30-1d gene product in translation initiation may involve interaction with ribosomal subunits.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Proteínas de Plantas
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Codón
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Proteínas Protozoarias
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Cloroplastos
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Regulación de la Expresión Génica
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Genes Supresores
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Chlamydomonas reinhardtii
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Genes Protozoarios
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Genes de Plantas
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Complejo de Citocromo b6f
Límite:
Animals
Idioma:
En
Revista:
Genetics
Año:
1997
Tipo del documento:
Article
País de afiliación:
Estados Unidos