PCR-RFLP typing of ureC from Helicobacter pylori isolated in Argentina from gastric biopsies before and after treatment with clarithromycin.
Epidemiol Infect
; 118(2): 119-24, 1997 Apr.
Article
en En
| MEDLINE
| ID: mdl-9129588
ABSTRACT
A clinical trial was conducted in Argentina to determine the efficacy of clarithromycin plus lansoprazole for the treatment of Helicobacter pylori in duodenal ulcers and non-ulcer dyspepsia. PCR-RFLP was conducted on an 820-bp amplified product of the ureC gene of H. pylori to determine the genetic heterogeneity of 83 pretreatment and 21 post-treatment isolates. Twelve different restriction patterns were observed when digested with Sau 3A or Hha I, resulting in 40 different RFLP types. Comparison of isolates before treatment to after treatment showed that 20 of 20 patients had the same RFLP type. In addition, the presence of the cytotoxin-associated gene (cagA) and the vacuolating gene (vacA) were determined. All pretreatment isolates were positive for vacA whereas 75% of the pretreatment isolates were positive for cagA. The results of this study indicate that a high degree of heterogeneity exists among H. pylori and that infection is not limited to a small number of RFLP types.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Gastropatías
/
Proteínas Bacterianas
/
Inhibidores de la Síntesis de la Proteína
/
Helicobacter pylori
/
Infecciones por Helicobacter
/
Claritromicina
/
Genes Bacterianos
/
Antibacterianos
/
Antígenos Bacterianos
Límite:
Humans
País/Región como asunto:
America do sul
/
Argentina
Idioma:
En
Revista:
Epidemiol Infect
Asunto de la revista:
DOENCAS TRANSMISSIVEIS
/
EPIDEMIOLOGIA
Año:
1997
Tipo del documento:
Article
País de afiliación:
Estados Unidos