Sulfide influence on polymorphonuclear functions: a possible role for Ca2+ involvement.
Immunopharmacol Immunotoxicol
; 19(3): 393-404, 1997 Aug.
Article
en En
| MEDLINE
| ID: mdl-9248866
ABSTRACT
Polymorphonuclear cells (PMN) of gingival sulcus play an important role in host defense against periodontal tissue-invading bacteria, but their phagocytic activity is conditioned by several virulence factors released by oral pathogens. In this report we have studied the influence of sulfide, a toxic bacterial metabolite, on the main PMN functions chemotaxis, degranulation and oxidative burst. PMN exposed to sodium sulfide (up to 2 mM) used as a source of H2S showed a depression of the calcium-dependent cytoskeleton activities such as chemotaxis and azurophilic granule release induced by FMLP. No effect was observed on the calcium-independent specific granule release obtained by PMA. These data were in agreement with the sulfide inhibition of cytosolic free Ca2+ concentration [Ca2+]i increase normally induced by ionomycin. On the other hand, hydrogen sulfide was able to prime PMN for a stronger oxidative response both to calcium-dependent or calcium-independent stimulation. This finding may account for a more efficient oxidative killing under reoxygenation of the anaerobic infectious areas.
Buscar en Google
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Sulfuros
/
Calcio
/
Neutrófilos
Límite:
Humans
Idioma:
En
Revista:
Immunopharmacol Immunotoxicol
Asunto de la revista:
ALERGIA E IMUNOLOGIA
/
FARMACOLOGIA
/
TOXICOLOGIA
Año:
1997
Tipo del documento:
Article
País de afiliación:
Italia