Effect of pretreatment of germanium-132 on Na(+)-K(+)-ATPase and galactose cataracts.
Curr Eye Res
; 16(8): 832-7, 1997 Aug.
Article
en En
| MEDLINE
| ID: mdl-9255513
ABSTRACT
PURPOSE:
Recently, we reported that topical administration of 2-carboxyethyl germanium sesquioxide (Ge-132) concurrently with 50% galactose feeding delayed the establishment of mature cataracts and reduced advance glycation product. This study was to determine the effect of pretreatment of Ge-132 on galactose associated morphological changes and Na(+)-K(+)-ATPase activity.METHODS:
Young Sprague Dawley rats received topical eye drops four times a day of either saline or Ge-132 seven days prior to the 50% galactose diet and during galactose feeding. At desired intervals the lenses were extracted, photographed and processed for either light microscopy, scanning electron microscopy or the determination of Na(+)-K(+)-ATPase activity.RESULTS:
In Ge-132 pretreated lenses as compared to saline pretreated lenses the following results were observed (a) the galactose-induced morphological alterations in the majority of lenses were delayed and (b) Na(+)-K(+)-ATPase activity was protected.CONCLUSIONS:
Our previous and current studies show that in addition to osmotic stress post-translational protein modification, such as glycation, including enzymes may play a role in initiating changes that lead to cataract development. The inhibition of protein glycation by antiglycating compounds, such as Ge-132, delays sugar cataract formation. Currently, we are investigating the status of protein glycation and advanced glycation end products following pretreatment with Ge-132 and the role of Ge-132 on the activities of enzymes such as aldose reductase and Na(+)-K(+)-ATPase.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Compuestos Organometálicos
/
Catarata
/
ATPasa Intercambiadora de Sodio-Potasio
/
Germanio
/
Cristalino
/
Antineoplásicos
Límite:
Animals
Idioma:
En
Revista:
Curr Eye Res
Año:
1997
Tipo del documento:
Article
País de afiliación:
Estados Unidos